Polyclonal Antibody to SNAI2

Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is therefore pleased to be able to offer researchers access to products that are not fully characterized or validated. IMGENEX IMX antibodies are shown by Peptide ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.

• We cannot guarantee that the IMX antibody will work in any application other than in a Peptide ELISA against the peptide used as immunogen and therefore can not offer a refund if the antibody does not work in your application.

• IMX antibodies are offered at a lower price compared to more highly validated antibodies.

• We are not able to provide the peptide used as immunogen for IMX antibodies.

As IMX antibodies are not fully validated we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.

Zinc finger transcription factor SNAI2/SNAIL2/Slug is found to have some functional significance in formation of axis and mesoderm in Drosophila. SNAIL2 and Twist act as negative regulators of level of Cerberus RNA, which encodes a Nodal, bone morphogenic protein (BMP) and WNT inhibitor. mRNA levels of SNAI2 are regulated by NF-kappaB in early embryo. Partial functional redundancy between Twist, SNAI2 and SNAI1 may be developmentally and physiologically significant given their roles in the induction and progression of various forms of epithelial–mesenchymal transition and the metastasis of cancer cells, the regulation of apoptosis, and their ability to generate stem cell like behaviors in immortalized human epithelial cells.

Antigen
Amino acids 82-100 (SLGRVSPPPPSDTSSKDHS) of human SNAI2 were used as the immunogen.