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Hardware | Turning the spotligh ...Turning the spotlight on recombinant proteinsTotal Word Count: 342 Author Rating: NA  Rate this article
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The proteins, peptides and lysates in our antibody catalog are as important to researchers as the antibodies themselves. Recombinant proteins are routinely used to study and analyze gene expression and protein synthesis in vivo, either alone or by the generation of antibody probes.
The proteins, peptides and lysates in our antibody catalog are as important to researchers as the antibodies themselves. Recombinant proteins are routinely used to study and analyze gene expression and protein synthesis in vivo, either alone or by the generation of antibody probes.
The recombinant proteins on our antibody database are produced using expression plasmids, or vectors, to generate a recombinant form of DNA which then expresses the recombinant protein via a host cell. To do this, a DNA sequence encoding the protein of interest is inserted into a prokaryotic or eukaryotic host cell, in a form which can be replicated and expressed as a recombinant protein. This is achieved by ligating the DNA into a specific expression vector or plasmid, containing all the genetic material required for the protein to be expressed independently of the host DNA. Both the vector and DNA are treated with restriction endonuclease enzymes, creating complimentary ‘sticky ends’ which can then base pair together. DNA ligase then covalently links the two DNA strands into one recombinant DNA construct. Using a transformation or transfection process, the DNA construct is then inserted into the host cell, which may be prokaryotic (bacterial) or eukaryotic (yeast, insect or mammalian), in order for recombinant protein expression to occur. Prokaryotic expression systems are low cost and fast, with a high yield. However, they are unsuitable for most eukaryotic studies for a number of reasons. Firstly the tendency to form insoluble inclusion bodies, which may interfere with the protein’s biological function. Secondly, the proteins are unable to undergo post-translational modifications; thirdly, eukaryotic introns will not be recognized, possibly leading to premature termination or improper folding during protein synthesis, resulting in a biologically inactive product. We at Novus Biologicals have a wide range of human and animal recombinant proteins on our antibody database, specific to a variety of cellular markers including proteases, tumor markers, adhesion molecules, cytokines and chemokines. Suitable for most assays, and fully translationally modified, they are produced to the same high levels of purity and performance as our antibodies. ArticleSource: ArticlesAlley.com
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