In Search of Putative FOXP3+ Cell Surface Markers

Despite intense interest and scrutiny focused on FOXP3 as a key protein & master transcription  factor, isolating and enriching for viable FOXP3 positive cells remains a challenge.  Although cell separation/staining via CD4+CD25+ selection is commonly used, this technique has limited applications. Thus, surface markers specific for FOXP3 positive cells would be invaluable research tools as they would:

•  Facilitate isolation & purification of viable

   Treg cells

•  Distinguish naturally occurring

    CD4+CD25+cells from both naive and

    recently activated CD4+CD25-

    nonregulatory T cells

•  Allow therapeutic manipulation of

    Treg cells

As the search for putative FOXP3+ markers continue, Neuropilin-1, GPR83, & FR4 have emerged as potential candidates. IMGENEX is excited to offer a panel of flow cytometric characterized antibodies against:

•  Neuropilin-1 (clone 211H6.01)

•  GPR83 (polyclonal)

•  Folate Receptor 4 (clones 12A5 & TH6)

Flow cytometric analysis of intracellular FOXP3 (IMG-5802D) and cell surface FR4 with clone 12A5 (IMG-6217C) (left) and clone TH6 (IMG-6218C) (right) at 0.06 ug/10^6 mouse splenocytes.

Flow cytometric analysis of Neuropilin-1 in CD4+CD25+ human PBMCs using A) an isotype control & B) DDX0440 at 0.5 ug/10^6 cells. These antibodies are available in multiple sizes and conjugates.